Determination of aconitase activity

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WebNov 11, 2003 · Determination of Aconitase Protein Levels and Activity in the Tomato Genotypes Having demonstrated that leaves of the Aco-1 plants exhibited lower … WebSomething that is curious about aconitase is the fact that it uses iron as the inorganic substituent. Mutanogenic studies on aconitase have found substituting the iron ions with …

Frataxin Acts as an Iron Chaperone Protein to Modulate …

WebJun 1, 2015 · The effect of pH on aconitase activity was studied in 100 mM tris–HCl buffer containing 40 mM citrate or 4 mM d, l-isocitrate. Determination of K i was performed by plotting the graphs of dependence of 1/v from the concentration of inhibitor [i] at two fixed concentrations of substrate (citrate) (Dixon, 1953). WebJun 23, 2024 · Prior studies indicated that H129 is the only histidine ligand involved in the control of the iron donation capability of YtfE, as its replacement for a leucine residue produces a protein unable to restore the aconitase activity . Interestingly, H129 forms an H-bond with E159 (H129 Nδ1 …E159 Oε1 2.66 Å). hierarchy of control for risk assessment

Biochemical elucidation of citrate accumulation in

WebJul 9, 2004 · Aconitase, a Krebs-cycle enzyme that converts citrate to isocitrate, belongs to the family of iron-sulfur–containing dehydratases whose activities depend on an intact cubane [4Fe-4S] 2+ cluster (1, 2).The purified enzyme is highly susceptible to oxidant-induced inactivation due to release of the solvent-exposed Fe-α and formation of a [3Fe … WebFor quantitative determination of aconitase enzyme activity and high-throughput screening assays for aconitase modulator. Key Features. Fast and sensitive. Linear detection range (20 µL sample): 0.5 to 100 U/L for 20 min reaction. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. WebDec 13, 2002 · Determination of activities of mitochondrial complexes. For complex activity measurements, samples of synaptosomes (1 mg) were incubated in 1 mL of standard medium for 20 min at 37°C with different concentrations of rotenone, antimycin, or cyanide, respectively. ... Aconitase activity was expressed as a function of the per cent … how far from antioch tn to lathrop ca

Oxidative damage during aging targets mitochondrial aconitase

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WebOct 14, 1997 · The aconitase activity was determined in the supernatant using two different methods. Method 1 is a coupled assay in which the formation of NADPH was … WebAlper Sarikaya, Aconitase 4 trans mechanism for the reversible dehydration of citric acid as well. From this determination, Gawron et al. surmised that the alpha-hydrogen of these substrates does not participate in the role of aconitase mechanism as the trans mechanism is nowhere near the proximity of the changing subunit. Additionally, the authors found … how far from antioch tn to redlands ca how far from antioch tn to aberdeen md

"WebMitochondrial aconitase is a reversible enzyme that catalyzes the conversion of citrate to isocitrate in the tricarboxylic acid cycle. Mitochondrial aconitase is very sensitive to oxidative... Determination of Aconitase Activity: A Substrate of the Mitochondrial Lon Protease Springer for Research & Development " - Determination of aconitase activity

Determination of aconitase activity

Web0.5M sorbitol at the concentration of 200mg/ml. Aconitase activity was determined by increase in absorbance at 340nm in the presence of 5mM citrate, 0.25mM NADP, 4mM MgCl 2, 10unit/ml of NADP-isocitrate dehydroge-nase (Murakami et al., 2006). 2.3. Determination of hydrogen peroxide and superoxide anion Determination of rosmarinic … WebThe aconitase activity was significantly depressed in the 2% O2 group (*P ⬍ 0.03, n ⫽ 6/group). ... Determination of the mechanism of free radical generation in damage in the presence of cyanosis: a new concept of controlled cardiac human aortic endothelial cells exposed to anoxia and reoxygenation. ...

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WebJan 10, 2024 · This protocol describes the determination of aconitase activity, which can provide an indirect measurement of Lon activity in vivo. The protocol is optimized for … WebAug 1, 2005 · Unanticipated, however, was the reduced expression of the tricarboxylic acid (TCA) cycle gene citB (encoding aconitase), resulting in the loss of aconitase activity and preventing the ...

WebSep 11, 2014 · In the aconitase activity assay protocol, aconitase catalyzes an equilibrium between aconitate, cis-aconitate and iso-citrate. These reactions are monitored by … WebTotal aconitase activity can also be measured in whole cell lysates, for example, 143B osteosarcoma lysate shown below (Figure 3). Note – the aconitase activity measured in …

WebAug 29, 2024 · First, in-gel activity assays showed that the mitochondrial aconitase activity (m-aco) in FRDA patient-derived cells increased significantly after SS-31 treatment. ... Determination of ... WebDetermination of Aconitase Activity—For the determination of the aconitase activity, cells from the CGXII main culture (∼25 ml) were harvested with ∼25 g of crushed ice (precooled to -20 °C) by centrifugation at 4,000 × g for 5 min. The cell pellet was resuspended in 900 μl of Tris-HCl (90 m m g; 4 °C), the supernatant was used ...

WebOct 1, 1974 · TAA is also known to play a role in the TCA cycle where it inhibits the aconitase (ACO), the enzyme converting citrate to isocitrate via cis-aconitic acid (CAA) intermediate [34] [35] [36 ...

WebThe Aconitase Activity Assay kit provides a simple and direct procedure for measuring Aconitase activity in a variety of samples. Aconitase activity is determined in a coupled enzyme reaction in which citrate is converted to isocitrate by aconitase. This results in a colorimetric (450 nm) product proportional to the enzymatic activity present. hierarchy of controls constructionWebAconitase activity determined after 15 hours incubation at 5', pH 7.4. effect. Phosphate thus competed with the protein for the iron in the system, as well as prevented the added … how far from armidale to dubboWebApr 14, 2024 · Plants of the genus Scutellaria (Lamiaceae) have a wide variety of bioactive secondary metabolites with diverse biological properties, e.g., anti-inflammatory, antiallergenic, antioxidant, antiviral, and antitumor activities. The chemical composition of the hydroethanolic extracts, obtained from dried plants of S. incarnata, S. coccinea, and … hierarchy of control in risk assessmentWeblevels of aconitase, whereas its overexpression decreases its levels and activity [15, 25] (Fig. 1). This protocol describes the determination of aconitase activity, which can provide an indirect measurement of Lon activity in vivo. … how far from arches to las vegasWebApr 10, 2024 · This piece explains an activity that allows for the simple and accurate determination of the heat of vaporization, ΔH vap, of water at 100°C, and ultimately the approximate strength of a hydrogen bond in boiling water, in kJ·mol –1. The vaporization of water is an endothermic process represented by Equation 1: how far from aqaba to petraWebDetermination of Aconitase Activity 4.5 ml. of water in each of a series of thick walled 100 X 14 mm. Pyrex test-tubes are brought to 30”. how far from aqaba to ammanWebAug 31, 1999 · The aconitase protein of Bacillus subtilis was able to bind specifically to sequences resembling the iron response elements (IREs) found in eukaryotic mRNAs. The sequences bound include the rabbit ferritin IRE and IRE-like sequences in the B. subtilis operons that encode the major cytochrome oxidase and an iron uptake system. IRE … hierarchy of controls 6 levels