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Web13 de mai. de 2024 · The expression levels of 13 genes were determined by qPCR using the Hieff UNICON qPCR SYBR Green Master in the Applied Biosystems 7500 System (USA) according to the manufacturer’s instructions. The PCR procedure was as follows: 5 min at 95°C, 40 cycles of 10 s at 95°C, and 40 s at 60°C, followed by melting curve analysis. Web1 de jul. de 2024 · The mRNA levels were assessed by qRT-PCR with the Hieff UNICON® qPCR SYBR Green Master Mix (YEASEN, Shanghai, China) in 20 μL reactions containing 200 ng of complementary DNA (cDNA) and 100 nM of each primer using an iQ5 Optical System (Bio-Rad, Hercules, CA, USA) following the manufacturer's instructions.
Hieff unicon
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WebProduct Description. Hieff Unicon TM Hotstart Direct Taq DNA Polymerase is a "hot start" DNA polymerase that tolerates blood and other repressors. This product is blocked by an … Web19 de jul. de 2024 · Fe (II) specifically accumulated in inflammatory-activated macrophages. THP-1 cells were differentiated into M0 macrophages through PMA stimulation and resting. The M0 cells were polarized to M1, M2a, M2b, M2c, and M2d cells by various stimulation methods. COU-LIP-1 was added to the cells at 10 μM to measure intracellular Fe (II) …
Web31 de jan. de 2024 · RT–PCR primers were set on exons adjacent to the processed intron to determine whether any alternative variants were produced. qPCR was performed to check whether there were any significant differences in expression in edited cells. qPCRs were processed using Hieff UNICON Universal Blue qPCR SYBR Green Master Mix (Yeasen) … Web30 de nov. de 2024 · The related primer sequences are shown in Table S1. qRT-PCR was conducted following the instructions for the Hieff UNICON® qPCR SYBR® Green Master Mix (Yeasen, Shanghai, China). The amplification system was 20 μL: Hieff UNICON® qPCR SYBR® Green Master Mix 10 μL, cDNA 1 μL, upstream and downstream primer 0.8 μL …
Web9 de out. de 2024 · Hieff UNICON® Universal Blue qPCR SYBR Green Master Mix用Universal ROX替代了传统ROX,弥补不同仪器平台的光程差,实现了适合所有qPCR仪器。另外,该产品采用抗体法修饰,同时配方添加了基因扩增的促进因子,大大提升了该产品的扩增性能。 产品特点 全仪器平台通用:预混特定类型参比染料,无需调整RO
Web10 de ago. de 2024 · Each sample was prepared in duplicate in a volume of 20 μL containing 10 μL of Hieff UNICON ® qPCR SYBR Green Master Mix (Cat No. 11198, Yeasen), 0.3 μL of each primer (10 ppm), 2 μL of genomic DNA, and 7.4 μL of ddH 2 O. fnp hessenWeb核心组分 Hieff UNICON ® Taq DNA聚合酶采用抗体法热启动,可以有效抑制样品准备过程中引物退火导致的非特异性扩增。 同时配方添加了提升PCR反应扩增效率因子和均衡不 … greenway indoor wall mount drying rackWeb本产品是2×Mix预混合试剂,能够实现在单个反应孔中进行多重荧光定量PCR。本产品含有基因改造的热启动Taq酶,极大地提高了扩增灵敏度和特异性,本产品对多重反应缓冲体系进行了深度优化,能够提高反应的扩增效率,促进低浓度模板的有效扩增。本产品可用于基因分型和基因多重定量分析。 greenway in concord ncWebHieff Unicon™ Universal Blue qPCR Master Mix (Dye Based) is a pre-solution for 2×real-time quantitative PCR amplification characterized by high sensitivity and specificity, is blue in color, and has the effect of sample addition tracing. greenway infinite campusWebProduct Description. The Hieff Unicon TM nCov Multiplex One Step RT-qPCR Probe Kit is a kit for performing multiplex quantitative PCR reactions using RNA as template. During … greenway indoor wall-mounted drying rackWebHFUN and UAV can be used through Wi Fi connection, including the following functions: 1. Connect to UAV Wi Fi; 2. It can watch the real-time pictures taken by UAV; 3. It can take … greenway industries of nj llcWebHieff Unicon™ Hotstart Direct Taq DNA Polymerase, 5 U/μL. Hieff UNICON™ Hotstart J-Taq DNA polymerase, 5 U/μL. Hieff Unicon™ Universal Multiplex qPCR Master Mix … greenway industries ct