Phis2.1 载体图谱

WebbDifferent pairs of plasmids (pGAD-OsWRKY54 and pHIS2- ProOsHKT1;5 , or control pGADT7 and pHIS2) were transformed into yeast cells. Transformed cells were spotted on SD medium containing 0, 50, or 75 mM 3-amino-1,2,4-triazole at 30 °C for 3 … WebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article …

Y187-pHis2YeastOne-HybridMediaKit 使用说明书

WebbPhis2osgpx1 Promoter Phis2 1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > TaKaRa > phis2 1. phis2 1 vector TaKaRa is a verified supplier TaKaRa ... shang government https://empireangelo.com

pHis2_载体及菌株库_产品中心_奥诺基因-HonorGene-基因研究资 …

http://www.biovector.net/product/319746.html WebbpHis2酵母表达载体说明- HaleWaihonaPukeBaidu 载体序列 ORIGIN 1 GAATTCCCGG GGAGCTCACG CGTTCGCGAA TCGATCCGCG GTCTAGAAAT ... pFLD/CAT pFLDα pGADT7-T pGAG424 pGAPZA pGAPZB pGAPZαB pGAPZαC pGBKT7 pGBKT7-53 pGBKT7-Lam pHIC-PI pHIL-D2 pHIL-S1 pHis2 pHisSi-1 pMETA pMETB pMETC pMETαA pMETαB pMETαC … Webb质粒名称：pHis2. 出品公司：Clontech . 质粒类型： 酵母单杂交系统. 克隆方法： 多克隆位点，限制性内切酶. 载体大小： 7207bp . 原核抗性： Kanamycin (卡那霉素) 真核抗 … shang grand tower address

酵母杂交实验常见问题——单杂知识及自激活检测篇_元件_-bait_作用

Category:T-Vector pMD19 (Simple) Sequence and Map - SnapGene

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Phis2.1 载体图谱

pHis2载体图谱质粒图谱、序列、价格、抗性、测序引物、大小等 …

Webb19 nov. 2012 · 我把要验证的转录因子构建到pGADT7-rec2载体上，与pHis2的空载共同转化Y187，本来要拿这个做阴性对照的，可是在三缺板子上长得很欢乐呀，加了3AT，并且 … WebbpHis2载体价格1000元，pHis2载体质粒图谱(Vector map),载体序列(Sequence)见下文，载体质粒抗性为Kanamycin，质粒大小为7207bp。pHis2是酵母单杂交载体的pHis2。

Phis2.1 载体图谱

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WebbT-Vector pMD19 (Simple) Linearized vector with no MCS and with a single 3'-T at each end, for TA cloning of PCR products and blue-white screening. Explore Over 2.7k Plasmids: … WebbpHIS2 Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for …

Webb1 sep. 2000 · The promoter cloned in pHIS2.1 is strong autoactivator and the yeast colonies transformed with this is growing even at 100mM 3-AT. However, the colonies having both constructs (protein cloned in ... WebbVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community …

WebbpHIS2 can be maintained in both yeast and bacteria. It contains an autonomous replication se-quence ( ARS4) and TRP1 nutritional marker for replication and selection in yeast (1, … WebbpHIS2 Vector Information PT3705-5 Sold as part of Catalog #K1617-1 Restriction Map and Multiple Cloning Site (MCS) of pHIS2 Vector. Unique restriction sites are in bold. *Note: The Sac II site is resistant to cleavage. Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding ...

Webb技术路线 1、文库质量评估：理论+涂板评价 交付材料：反馈质量评估结果 2、载体构建：基因合成诱饵基因 构建Phis2载体 交付材料：含诱饵基因的质粒 3、启动子背景筛查：Binding seq基因启动子的背景筛查 交付材料：阳性克隆测序结果 word项目报告 4、文库筛选：诱饵质粒转化和文库质粒转化 酵母筛选和3AT条件优化 筛选结果测序 交付材料：阳性克隆测 …

Webb19 sep. 2024 · C-repeat binding factors/dehydration responsive element binding factors 1 (CBFs/DREB1s) are a small family of transcription factors that play important roles in plant resistance to various external stresses. However, functional characterization of tea plant (Camellia sinensis L.) CBF gene (CsCBF) was still seldom reported. Here, functional … shang grand tower contact numberWebb16 mars 2024 · 诱饵载体：pHis2-DNA（筛选标记Trp报告基因His） 文库载体：pGADT7-cDNA（筛选标记Leu） 实验步骤： 一、转化诱饵载体（参考SK2400中质粒转化步骤） 1. YPDA平板培养划线，活化Y187酵母菌种；挑酵母菌落于液体YPDA培养基摇菌培养； 2. 制备感受态并将pHis2-DNA转化进入感受态细胞； 3. SD/-Trp平板检测转化结果。 二、自 … shang government systemWebb16 jan. 2015 · 广东汕尾市海丰县自然资源局公开招聘政府聘员1人【含答案解析】模拟试卷8 未来工匠我来说演讲稿范文（5篇） 2024年山东潍坊寿光市事业单位招考聘用硕士研究生30人【答案解析】模拟试卷5 shanghai098 jt111.comWebbpHis2酵母单杂交载体是由Biovector Co.,LTD代理或销售的Biovector, Addgene, ATCC, Invi品牌的试剂，产品来源于Biovector Inc. USA。Biovector Co.,LTD是中国最权威的pHis2酵母单杂交载体试剂销售服务商之一，在海淀区等地方销售pHis2酵母单杂交载体试剂已经多年。生物在线为您提供众多企业pHis2酵母单杂交载体仪器产品及 ... shang guan sha traduction francaisWebb26 maj 2024 · 质粒图谱 载体描述 pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was … shanghai07.comhttp://www.biofeng.com/zaiti/jiaomu/pHis2.html shanghai 114 reissuehttp://www.bioon.com.cn/doc/showarticle.asp?newsid=101857 shanghai 14 day weather forecast