Phospho mtor antibody

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August undefined, 2024

WebMammalian target of rapamycin (mTOR) is a serine/threonine kinase that plays a key role in cell growth, cell proliferation, and protein synthesis. mTOR mediates phosphoinositide 3 … WebMTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor …

Phospho-mTOR (Ser2448) Antibody Affinity Biosciences

WebA synthetic phospho-peptide corresponding to residues surrounding Ser2448 of human phospho mTOR. Formulation. 1X PBS, 0.09% NaN3, 0.2% BSA. Isotype. Rabbit IgGk. Preparation. Protein A+G. Recommended Usage. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. WebDec 28, 2024 · Phospho-mTOR (Ser2481) Antibody Catalog Number: 2974S Clone Number: N/A Info: Supplier Page View Product Specs Browse Similar Products: MTOR Antibodies … cryovac trays for meats and produce

Phospho-mTOR (Ser2448) antibody (CL594-67778) Proteintech

WebWestern blot analysis of NIH/3T3, using Phospho-mTOR-S2448 Rabbit mAb (AP1413) at 1:1000 dilution.NIH/3T3 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in … Web4% PFA plus methanol. Methanol only. This clone failed to immunoprecipitate EGFR Phospho (Tyr845) from whole cell extracts prepared from EGF-stimulated A-431 cells. This recombinant antibody clone is derived from clone A18028A. This clone is predicted to recognize the region corresponding to EGFR Phospho (Tyr845) in both rat (Tyr846) and … WebThe Phospho-mTOR (Ser2481) Antibody from Cell Signaling Technology is a Rabbit Polyclonal antibody to MTOR. This antibody recognizes Human, Mouse, Rat, and Non-Human Primate antigen. The Phospho-mTOR (Ser2481) Antibody has been validated for the following applications: Western Blot. duoapp.otiw.org

Phospho-mTOR (Ser2481) Antibody from Cell Signaling Technology …

Anti-p-mTOR Antibody (59.Ser 2448) SCBT - Santa Cruz Biotechnology

WebMTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal ... WebRabbit Phospho-mTOR-S2448 Rabbit mAb (AP1413), validaed in WB,IHC and tested in Human,, Mouse. 100% Guaranteed. ABclonal provides trial size antibody samples for target detection. Replacement to Abcam, Santa Cruz, Sigma and CST antibody. duo app for microsoft edgeWebindicates those antibodies for which a 10 µg trial size SAMPLE is available free of charge. indicates antibodies with CONJUGATED forms (AC, HRP, PE, FITC, Alexa Fluor® 488, 546, 594, 647, 680 and 790) available. mTOR Antibodies Phospho-Specific mTOR Antibodies mTOR CRISPR Knockout, HDR and Nickase Knockout Plasmids mTOR CRISPR Activation … dúo arenal facebook

"WebThis Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human and mouse phospho-AKT/ GSK3 beta / mTor and total-AKT/ GSK3 beta / mTor. For each target, a capture antibody has been coated onto microwells. " - Phospho mtor antibody

Phospho mtor antibody

Phospho-mTOR (Ser2448) Monoclonal Antibody …

WebMTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor … WebRabbit recombinant monoclonal mTOR (phospho S2448) antibody [EPR426(2)] - BSA and Azide free. Validated in WB, IHC, Dot and tested in Mouse, Human.

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WebThis affinity purified antibody has been tested for use in immunohistochemistry, ELISA and western blotting. Western blotting shows reactivity specific for phospho mTOR detecting a band at approximately 250 kDa. Reactivity in other immunoassays is unknown. Purity/Specificity WebPhosphoPair mTOR (Ser2448) Antibody Set. Whole cell extracts (15 µg protein) from serum starved HeLa and NIH/3T3 cells treated with 10 µM rapamycin (Rap) for 2 hours or with 150 nM insulin (Ins) for 6 minutes were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 ...

Webtracts were immunoprecipitated with rabbit -mTOR antibody or-AU1 antibody. Immune complexes were collected on protein A-Sepha-rose (Sigma) or -mouse IgG-agarose (Sigma), washed three times with ... tion—Previous reports demonstrated that mTOR was phospho-rylated in vitro by AKT at a serine residue (Ser-2448) located in studies, we ... WebApr 14, 2024 · Neuropathic pain caused by trigeminal nerve injury is a typical refractory orofacial chronic pain accompanied by the development of hyperalgesia and allodynia. We previously demonstrated that the mammalian target of rapamycin (mTOR) inhibitor rapamycin suppressed orofacial formalin injection-induced nociception; however, the …

WebFor Analyzing Cell Signaling Pathway Activation. Phosphorylation, the addition of a phosphate group to serine, threonine or tyrosine residues, is an important cellular process utilized to send cellular signals from the membrane to the nucleus. For example, changes in physiological conditions may require immediate changes in gene expression. WebAnti-phospho-mTOR (Ser2159) Antibody Anti-mTOR Antibody detects level of phospho-mTOR (Ser2159) & has been published & validated for use in Western Blotting, Peptide Inhibition Assay, Dot Blot. - Find MSDS or SDS, a COA, data sheets and more information.

WebProduct name Anti-mTOR (phospho S2481) antibody [EPR427 (N)] See all mTOR primary antibodies Description Rabbit monoclonal [EPR427 (N)] to mTOR (phospho S2481) Host species Rabbit Tested applications Suitable for: WB, Dot blot more details Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP Species reactivity Reacts with: Human

WebAnti-phospho-mTOR (Thr2446) Antibody Upstate®, from rabbit; Synonyms: FRAP1, FRAP, FRAP2, RAFT1, RAPT1, FK506 binding protein 12-rapamycin associated protein 1; find … duo app not working on new phoneWebmTOR is a member of the PI3 family of kinases and plays a critical role in regulating cell proliferation and metabolism in response to nutrient availability, cellular energy status, … duo apple watch pushWeb2884) and total mTOR antibodies were diluted 1:1000. Phospho-S6K (Thr-389), Phospho-S6K (Thr-421/424), total S6K1, phospho-4E-BP1 (Thr-37/46), and total 4E-BP were diluted 1:1000 (Cell Signaling Technology, Beverly, MA). β-actin antibody was diluted 1:5000 and FKBP12 antibodies were diluted 1:200 (Santa Cruz, Santa Cruz, CA). Anti-rabbit and ... duo astral plane beach crazyWebDownload Zoom Anti-mTOR Antibody is an antibody against phospho-mTOR (Thr2164) for use in Dot Blot, Western Blotting. Anti-phospho-mTOR (Thr2164) Antibody MSDS … cryovac vacuum packaging machinesWebMTOR (2475) Expression: P42345 MTOR_HUMAN: Expressed in numerous tissues, with highest levels in testis. Description: an atypical kinase belonging to the PIKK family of kinases. Controls cell growth through protein synthesis regulation. Downstream of PI3K/Akt pathway and required for cell survival. duo arthur hWebApr 14, 2024 · Neuropathic pain caused by trigeminal nerve injury is a typical refractory orofacial chronic pain accompanied by the development of hyperalgesia and allodynia. … cryovac vffsWebMTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal ... duo app on new phone