Phospho mtor ser2448

WebJan 15, 2009 · mTOR phosphorylated at S2448 can be found in a complex with both, raptor and rictor. Total lysates of logarithmically growing HEK293 cells were prepared and used for immunoprecipitation of endogenous raptor or rictor. WebFeb 12, 2024 · We measured the levels of mTOR phosphorylation at Ser2448, and the mTOR target protein 49, the ribosomal protein S6 kinase (p70S6K) phosphorylation at Thr389, …

ab168538 – Kit mTOR (pSer2448) ELISA - Abcam

WebThe MSD Phospho(Ser2448)/Total mTOR Assay is available on 96-well 4-Spot plates. This datasheet outlines the performance of the assay. Typical Data Representative results for the Phospho(Ser2448)/Total mTOR Assay are illustrated below. The … WebMammalian target of rapamycin (mTOR) is a serine/threonine kinase that plays a key role in cell growth, cell proliferation, and protein synthesis. mTOR mediates phosphoinositide 3-kinase and Akt/PKB signaling, resulting in phosphorylation … chrome pc antigo https://empireangelo.com

Phospho-mTOR (Ser2448) Monoclonal Antibody …

WebMammalian target of rapamycin (mTOR) is a serine/threonine kinase that plays a key role in cell growth, cell proliferation, and protein synthesis. mTOR mediates phosphoinositide 3 … WebProduct Description. The PathScan ® Phospho-mTOR (Ser2448) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects … WebPhospho-mTOR (S2448) and Total mTOR ELISA Kit (ab279869) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated mTOR protein in your experimental model system, you can verify pathway activation in your cell lysates. chrome pdf 转 图片

mTOR phosphorylated at S2448 binds to raptor and rictor

Category:MSD Phospho( Ser2448 )/Total mTOR Assay - Meso Scale

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Phospho mtor ser2448

Phospho-mTOR (Ser2448) Recombinant Rabbit Monoclonal …

WebThe Phospho-mTor (Ser2448) assay measures mTor when phosphorylated at Ser2448. Contrary to Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis or transfer. The Phospho-mTor (Ser2448) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor. WebPrinciple of Assay. This mTOR (phospho Ser2448) Cell Based ELISA Kit allows for the detection of mTOR (phospho Ser2448) and the effects that certain stimulation conditions have on mTOR (phospho Ser2448) expression in different cell lines. Qualitative determination of mTOR (phospho Ser2448) concentration is achieved by an indirect …

Phospho mtor ser2448

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WebWe show that Ser-2448 is directly phosphorylated byp70S6 kinase in vitro and that expression of a rapamycin-resistantp70S6 kinase in cells maintains Ser-2448 phosphorylation in the presence ofrapamycin. Furthermore, changes in amino acid availability, which affectsp70S6 kinase activity, also modulates Ser-2448 phosphorylation. WebPhosphoPair mTOR (Ser2448) Antibody Set. Whole cell extracts (15 µg protein) from serum starved HeLa and NIH/3T3 cells treated with 10 µM rapamycin (Rap) for 2 hours or with …

WebThe mTOR subunit of mTORC1 and 2 is phosphorylated at Ser2448 in a PI3/Akt- signaling-dependent manner; this post-translational modification is associated with increased … WebProduct Description This peptide is used to specifically block #2971 Phospho-mTOR (Ser2448) Antibody reactivity. Quality Control The quality of the peptide was evaluated by …

WebmTOR (phospho Ser2448) stained by mTOR (phospho Ser2448) antibody (GTX132803) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min GTX132803 WB Image Untreated (–) and treated (+) 293T whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR (phospho Ser2448) antibody … WebThe MSD Phospho(Ser2448)/Total mTOR Assay is available on 96-well 4-Spot plates. This datasheet outlines the performance of the assay. Typical Data Representative results for …

WebRayBio® Human and Mouse Phospho-mTOR (Ser2448) and Total mTOR ELISA Kit. This assay semi-quantitatively measures mTOR phosphorylated at Serine-2448 as well as total mTOR in cell lysate samples. $567.00 CODE: PEL-mTOR-S2448-T-1 Number of Plates: Quantity: + − Add To Cart View all product options Description Reviews Ask a question …

WebMonoclonal Antibody for studying mTOR (Ser2448) phosphate. Cited in 213 publications. Validated for Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and … chrome password インポートWebAug 24, 2005 · Anti-mTOR (phospho S2448) antibody [EPR426 (2)] (ab109268) Research with confidence – consistent and reproducible results with every batch Long-term and scalable supply – powered by … chrome para windows 8.1 64 bitsWebInvitrogen Anti-Phospho-mTOR (Ser2448) Monoclonal (MRRBY), eBioscience™, Catalog # 12-9718-42. Tested in Flow Cytometry (Flow) applications. This antibody reacts with … chrome password vulnerabilityWebMonoclonal Antibody for studying mTOR (Ser2448) phosphate. Validated for Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, … chrome pdf reader downloadWebInvitrogen Anti-Phospho-mTOR (Ser2448) Polyclonal, Catalog # 44-1125G. Tested in Western Blot (WB) and Immunocytochemistry (ICC/IF) applications. This antibody reacts … chrome pdf dark modeWebMonoclonal Antibody for studying mTOR (Ser2448) phosphate. Cited in 1811 publications. Validated for WB, IP, IF. Available in 2 sizes. Highly … chrome park apartmentsWebA synthetic phospho-peptide corresponding to residues surrounding Ser2448 of human phospho mTOR. Formulation. 1X PBS, 0.09% NaN3, 0.2% BSA. Isotype. Rabbit IgGk. Preparation. Protein A+G. Recommended Usage. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. chrome payment settings