Web本製品は、短時間で高い形質転換効率が得られるように調製されたサブクローニンググレードの大腸菌dh5αコンピテントセルです。 従来のコンピテントセルでは、形質転換 … WebDH5α Competent Cells are suitable for variety of applications requiring high transformation efficiency: • Efficient DNA cloning derived from PCR, cDNA-generation reactions • recA1 marker facilitates working with difficult to transform DNA • Ideal for generation of cDNA libraries using plasmid-derived vectors • Site-directed mutagenesis Genotype
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WebApr 13, 2015 · Treatment with 1-methyl-4-phenylpyridinium ion (MPP + ), rotenone (Rote), or antimycin A (Anti A), inhibitors of mitochondrial OXPHOS, facilitated glucose consumption at 24–72 h ( Fig. S1 A) and increased intracellular pyruvate at 8 h ( Fig. S1 B) and extracellular pyruvate at 24–72 h ( Fig. S1 C ), indicating that inhibition of OXPHOS activated … WebMAX Efficiency DH5α T1 Phage-Resistant Competent Cells are high efficiency chemically competent cells for generating cDNA libraries. In addition to supporting blue/white screening, recA1 and endA1 mutations in DH5α increase insert stability and improve the quality of plasmid DNA prepared from minipreps.MAX Efficiency DH5α T1 Phage … bottle gas refill near me
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WebPlasmid DNA constructs were transformed into high efficiency DH5α competent cells (NEB) for propagation, and purified by the GeneJET plasmid miniprep kit (Thermo-Scientific Rockford, IL, USA), following the manufacturer’s instructions. For cell transfection, RAW264.7 cells were transfected with PRKCB-REP, an empty REP vector using TurboFect ... WebDescription. Library Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that demonstrate transformation efficiencies of >1 x 10 8 cfu/μg plasmid DNA. Supports blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal. Maintains plasmid DNA with minimum recombination events. WebSubcloning Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that provide a transformation efficiency of > 1 x 10 6 cfu/μg plasmid DNA. Designed for general, everyday use Contain genetic markers useful for general applications Not high-throughput compatible (Manual) Cloning, Transformation Order Info hayloft 2 on guitar